DryADD™ series is dried life-science reagent which is using new drying technology as a concept “from Labo to Field”. It is stable at room temperature, no need for refrigerated shipping or storage realization of the diagnostics suitable for the on-site testing. You can bring DryADD™ “from Labo to Field”.
Phytoplasma Universal Detection LAMP Kit
We can supply paid sample for 8 tests. Please contact us.
DryADD™ technology is original method which is developed by Nippon Gene Material.
It makes possible;
|▪ Shipping and storage at room temperature
|No need of refrigerator and dry ice
|▪ All-in-one dried reagent in a tube
|No need of preparation of reaction mixture
|Easy and simple
|Reduce contamination risk and human error
DryADD™ Phytoplasma Universal Detection LAMP Kit offers detection of all Phytoplasma in the class Mollicutes inclusively from plant samples using Loop-mediated Isothermal Amplification (LAMP) method. In this kit, a part of Phytoplasma DNA amplifies using LAMP method, and infection of Phytoplasma can be judged if the amplification occurs or not.
The operation needed for the test is extremely easy, simply to mix the DNA extract from plant sample with the test solution (dissolved Phytoplasma Detection Dry Reagent), and keep the solution at 64 degrees Celsius for 60 minutes.
For detection of DNA amplification, this kit utilizes visual inspection of fluorescence emitted from the solution after the whole reaction, so that the DNA amplification and detection can be done in one closed tube. Therefore, the amplification of Phytoplasma DNA can be detected safely in a short period of time.
Hydrangea Infected with hydrangea phyllody disease Caused by Phytoplasma
Phytoplasmas are plant pathogenic bacteria hosting on the phloem of plants, which were first discovered in Japan, 1967. They infect over 700 plant species worldwide, cause numerous symptoms as yellows, stunt, dwarf, proliferation, phyllody, witches’ broom, and are transmitted by insect vectors to many economically important crops, fruit trees and ornamental plants.
In order to prevent the devastating damage to the plant, it is necessary to provide effective means for controlling Phytoplasma such as removal of infected plant. However, since Phytoplasma is difficult to cultivate, it has not been provided the convenient way to diagnose the Phytoplasma infection. This kit can detect Phytoplasma inclusively, with no means of difficult operation such as conventional culture methods.
LAMP (Loop-mediated Isothermal Amplification) method allows the whole reaction process, including denaturing, to proceed at a constant temperature in an incubator. Thermal cycling machine is not needed for this kit.
Please refer to the homepage of Eiken Chemical Co., Ltd. about the detailed principle of LAMP method.
Eiken GENOME SITE; https://loopamp.eiken.co.jp/en/
|Phytoplasma Detection Dry Reagent
|48 tubes (8 well x 6 strips)
|Phytoplasma Reagent Dissolve Solution
|400 µl x 6 tubes
|Phytoplasma Extraction Solution
|1 ml x 48 tubes
|Phytoplasma Positive Control
|Phytoplasma Control Dissolve Solution
|400 µl x 1 tubes
|Phytoplasma Negative Control
|400 µl x 6 tubes
|400 µl x 6 tubes
* Please do not proceed all procedure at the same time, carry out each process in order of A → B → C.
coconut wood chip (0.005g)
A-1. Take out the required number of Phytoplasma Extraction Solution tube.
A-2. Scale 0.005 g of coconut wood chip from stem and soak it completely in Phytoplasma Extraction Solution.
A-3. Invert to mix upside down, then incubate the microtube at 95˚C for 10 minutes.
A-4. Invert to mix upside down, then cool with water to room temperature (the solution obtained is DNA sample).
B-1. Take out one of Phytoplasma positive control tube.
B-2. Add 10 µl of Phytoplasma Control Dissolve Solution.
B-3. After spinning down, leave it at room temperature for 5 minutes.
B-4. After vortexing, spin down the tubes (Phytoplasma Positive Control Solution).
C-1. Take out the required number of Phytoplasma Detection Dry Reagent.
C-2. Add 23 µl of Phytoplasma Reagent Dissolve Solution to each tube.
C-3. Add 2 µl of sample, close the cap, and spin down.
C-4. After 2 minutes, overlay with 20 µl of Mineral Oil.
C-5. Incubate test tube at 64˚C for 60 minutes (test reaction).
C-6. Terminate the reaction at 80˚C for 2 minutes.
Eiken Chemical Co., Ltd. owns the patent right for execution of Loop-mediated Isothermal Amplification. Nippon Gene Material Co., Ltd. has been granted the license to develop, manufacture and sell the kit for Phytoplasma detection.
*The link jumps to "Contact us" of Nippon Gene Co., Ltd. portal site which is introducing plant disease testing kits and services providing by the Nippon Gene Group.